KMID : 0613820100200020253
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Journal of Life Science 2010 Volume.20 No. 2 p.253 ~ p.259
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Neuroprotective Effects of Methanolic Extracts from Peanut Sprouts
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Kim Hyun-Jung
Kang Jum-Soon Park Hae-Ryong Hwang Yong-Il
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Abstract
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The neuroprotective effects of extracts from various parts of peanut sprouts on glutamate-induced neurotoxicity in N18-RE-105 cells were investigated. This study was performed to evaluate the neuroprotective activity of methanolic extracts from the whole (WME), heads (HME), and stems (SME) of peanut sprouts. The neuroprotective effects of these extracts were measured by MTT reduction assay, LDH release assay, phase-contrast microscopy, and flow cytometric analysis on the N18-RE-105 cells. Among these extracts, the HME showed the greatest neuroprotective effects, and was further fractionated with hexane, diethyl ether, ethyl acetate, and water, according to degree of polarity. Out of the fractionated extracts, the diethyl ether layer showed the highest activity on glutamate-induced cytotoxicity in N18-RE-105 cells. The sub-G1 DNA contents of the glutamate-induced severely apoptotic N18-RE-105s were measured by flow cytometric analysis to confirm the HME¡¯s anti-apoptotic activity. Interestingly, after incubation with 100 §·/ml of the HME, the proportion of sub-G1 cells of the glutamate-stressed N18-RE-105s had been greatly reduced, from 58.5% to 9.1%. These results imply that HME may have strong potential as a chemotherapeutic agent against neuronal diseases.
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KEYWORD
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Neuroprotective, N18-RE-105 cells, peanut sprout, apoptosis
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